>EMIYA
Tracing Harpe would be enough. No unnatural regeneration allowed, and Spartacus's suicidal strategy causes him to just lose his flesh cut by cut.
>Medea
No magic resistance? She will teach him the new meaning of "oppressor" with her gravity field and destruction lasers. It's just impossible.
>Seiba
Excalibur would destroy him without doubt, but without using that, it'd be a very hard fight. She's bad with durable foes.
>Regend
He'd have to settle it decisively with Tsubame Gaeshi before Spartacus has transformed too far. However, his great skill might just be enough to claim Spartacus's head before needing anything else. Kojirou does nothing but lethal attacks, so if Spartacus does his "let me be injured so I can really fight" deal, he's doomed...
>Hassan
Another instant killer. Hopeless.
>Meduseless
E rank magical energy. Petrified before he can do anything.
Spartacus has his points, but the 5th Holy Grail War is full of monsters. He is not even close to the same league.